The Weizmann Institute of Science's molecular genetics experts have developed a mechanism to culture human stem cells more faster than previously possible.
The researchers revealed that very early human cells may be produced and effectively incorporated into mice in a study published in the journal Cell. This is due to their undifferentiated, or naive, nature, in which they can develop into any form of cell in the body, including other stem cells, according to researchers. They've also discovered that the stem cells they generated are far more competent, allowing them to mix and integrate better with their host environment.
This greatly increases the odds of developing a cross-species chimera, which allows cells from one creature to help another grow. The researchers have found a strategy for increasing the efficiency with which these cells can combine, and their findings could be used to transplant cells or organs from one animal to another, or even from humans to other species.
tracing the research
Prof. Jacob Hanna and his colleagues created history in 2021 when they infused human stem cells into mice for the first time and demonstrated that they can successfully integrate into growing mouse embryos. After eight years, the researchers sought to expand their research by attempting to create an even earlier variety of stem cells that might be employed in similar treatments.
The researchers turned off two more signaling pathways to overcome a variety of challenges, resulting in naive human stem cells with a stable genome, few gene regulation errors, and, most importantly, the ability to differentiate flawlessly. They also changed a crucial gene involved in genome integrity, resulting in stem cells that are both competent and competitive, meaning they can integrate without harming the host. According to Hanna, this boosted "the chances of a successful transfer by around fivefold compared to what we were able to do in the past."
"If we ever want to produce a pancreas in pigs for human transplantation, for example," Hanna explained, "we'll have to consider these vast evolutionary disparities between species, starting with mice and humans." While it will be some time before we are able to create human organs in the lab for transplantation, this stem cell research has brought us one step closer.
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